IUBio

Best Method for Freezing tissues for RT-PCR?

Chris Martin Hodgson Chris.Hodgson at mcmail.com
Thu Jan 8 21:47:06 EST 1998


I agree with Mark - lyse in a chaotropic buffer/reagent and then rapidly
freeze the lysate - I used to do this with whole lymph nodes and
isolated or cultured cells. 

At my convenience, I then processed the lysates to generate RNA from
multiple experiments.

Best wishes

Chris H




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