I was wondering whether anyone has had problems with nonspecific binding
of newborn primate antibodies. I use Immulon II plates with a
baclovirus antigen and then blocked with 3%NonFat Dry Milk/PBS/.05%x-100.
For some reason I still get nonspecific binding even at 1:100 dilution of
these known seronegative animals. They no longer bind nonspecificly at 2
weeks post innoculation. Could it be something in the plasma like IgM or
some protein. The second antibody is anti-IgG primate and so I doubt it is
IgM. If you have any suggestions as to what might be the cause, please
let me know.
thanks
D. Verhoeven