IUBio

Sodium Azide and HRP

Pete immuno-affinity at rocketmail.com
Mon Jan 19 10:17:32 EST 1998


rwaldo at ARCHES.UGA.EDU (Krause Lab 1) wrote:

>I am curious as to why Sodium Azide cannot be used in buffers when using 
>HRP conjugates. Apparently Alkaline phosphatase development isn't
>affected by this.
>Thanks in advance for this information,
>Jarrat Jordan- UGA micro

Azide inhibits the enzymic action of HRP >>> No colour!!

Pete



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