John Ladasky wrote:
> O.K., so you're suggesting that when I see healthy-looking, active-
> ly-clumping cultures at the end of a week, I should provide PBMC and target
> cells, but withhold the IL-2? I could give this a try, but I'm a bit sur-
> prised. Some of the protocols I've seen call for 50-100 U/ml IL-2, and I'm
> only using 20 U/ml. Of course, one other protocol calls for 10 U/ml IL-2
> and 50 U/ml IL-4...
>> Well, it's official -- the 48-well culture went quiescent at the
> beginning of last week and did not restimulate this week.
I hope your lines are strugglin' along. The reason for the suggestion about
the il2 is in reference to activation in duced apoptosis that has been
elucidated by ab. abbas for one. Have you tried the rest steps (?) maybe
IL-4 instead of IL2, I've never wanted long-term cells so my suggestions are
really guesses.
markH
