I'm having trouble getting positive western blot results on an anti-body
found in patient serum. The anti-body was evident in immunohistochemistry on
rat tissue but did not show up in westerns when screened against cortex and
purkinjie proteins. I have tried SDS-Page and am attempting non-denaturing
non- reducing gels. Has anyone encountered similar difficulties or has any
suggestions. (non-denaturing, non-reducing protocols) or does anyone have any
other suggestions.
Thanks
Shennan Weiss
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