IUBio

help-extinction coefficient

Jay Mone' jmone at MARAUDER.MILLERSV.EDU
Fri Jul 17 09:25:13 EST 1998


Extinction coefficients are named according to the unit of 
concentration contained within them.  For example, a molar extinction 
coefficient is the absorbance of a 1M solution of the compound at a 
specified wavelength (usually the lamda maximum) in a 1 cm cell.
Extinction coefficients can be determined by plotting as standard 
curve of the compund at a given wavelnegth, and then finding the slope 
of the line.  This value is very important in determining the 
concentration of the compound yu are measuring, according to the 
Beer-Lambert law A = e l c, where A is absorbance, e is the extinction 
coefficient, l is the path length, and c is the concentration.
Hope this helps.

Jay Mone'



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