Colleagues:
We are trying to use the method of Boon et al to discover new class I
restricted epitopes/antigens. This method involves transfecting Cos 7
(AFrican Green Monkey) cells with human class I heavy chain; it is
expressed functionally on the cell surface with monkey B2M and peptide. In
any case we need a mAb that will stain all or "most" human class I
alleles, but NOT stain african green monkey cell surface class I. In
preliminary work we have used allele-specific mAb that bind only our
positive control human class I, but we are now working with a rare class I
allele (B45) which does not have a allele-specific mAb I know of. So we
need a broadly reactive mAb for human class I that does not bind monkey.
Any leads would be appreciated. David KOelle viralimm at u.washington.edu
David Koelle MD
Assistant Prof. Allergy/Infectious Diseases University of Washington
phone 206 667 6807 fax 206 667 4411
UW internal mail: "mail stop 358080 Room D3-100"
outside mail: Fred Hutchinson Cancer Research Center Room D3-100
1100 Fairview Ave N
PO Box 19024
Seattle, WA 98109-1024
