I experience non-specific binding in western-blotting system of mouse tissue
samples
My primary Abs are immunoaffinity purified rabbit Ab against pY, ERK1, P38
(hog1) and p53. All my blots contained nastic bands corresponding to
immunoglobulins. I thought it is attributed to the cross-reactivities of
anti-rabbit Ig's to mouse Igs. But the bands are intensified through
increasing the conc. of primary Ab but not the secondary. Pleasae help.
