I have used 20 mcl alamarblue, 5x10**5 cels/ml, 200 mcl cellsusp.(human
lymphocytes - PHA stimulated). The cells were preincubated for 48 hours and
the alamarblue added hereafter and meassured at 570 and 600 nm after 2, 4,
6 and 24 hours on a microtiterplate reader.
No significant difference was seen between the PHA stimulated and the basal
cells.
The assay is functioning with 3-h thymidine.
I would be greatfull if you have any coments concerning using alamarblue .
Yours Jens R. Hansen
