IUBio

biotinylation of DNA

mark mark.haynes at mail.tju.edu
Wed Jun 24 11:43:59 EST 1998


Devon Taylor wrote:
> 
> I am trying to find a kit or an economical source to biotinylate DNA for
> FACS analysis on SLE auto immune B-cells.
> Any suggestions will be appreciated


any number of companies sell kits to biotinylate the standard methods are 
random prime labelling, nick translation, or that good ol PCR reaction 
the biotin is incorporated with the use of a biotinylated uridine 
molecule.
markH



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