IUBio

IgM purification

Bjorn P. eped at online.no
Sun Mar 15 10:39:07 EST 1998


I need to purify some mouse anti-human antibody of IgM subclass. Until
> now, I have only been working with antibodies of IgG subclass. I have
> tested the binding of this IgM-antibody to Protein A, and found that
> some other column material has to be used. Any suggestions for
> protocols? Column material, buffers, pH's etc.

Bjorn
eped at online.no




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