David Verhoeven wrote:
>> I isolate PBMCs from blood utilizing a Ficol gradient. The only problem
> is that I still get comtamination with platelets. Is there a way to get
> rid of the platelets from my PBMCs?
A way to deplete platelet is low rpm centrifugation (800 rpm) but you
will also loose many PMNC. I rutinelly work with PMNC, doing flow
cytometry, immunohistochemistry and cell culture. I never had problems
because of platelets around.
Corrado
Dept Cell and Mol Biol
Univ Umea, Sweden
