An additional step to low-speed spin is to layer very gently 10-15 ml of the
cell suspension over an equal vol of fetal calf serum. Centrifuge 800 rpm,
10 min at 15o. This may help in decreasing the contaminating platelets.
David Verhoeven wrote:
> I isolate PBMCs from blood utilizing a Ficol gradient. The only problem
> is that I still get comtamination with platelets. Is there a way to get
> rid of the platelets from my PBMCs?
