I am trying to wake up hybridoma cells produced by the fusion of mouse
splenocytes to myeloma cells (Sp2/O-Ag14). The hybridoma cells were selected
for HGPRT+ and cryopreserved at -80C almost 2 years ago. Since this is my
first time working with hybridoma cells, I would appreciate any guidelines
for how I can tell if the cells have been succesfully revived (morphology,
growth rate, etc.). They appear to be growing very slow, for they do not
reach confluency after a month of growth in 5% CO2, 37C, in Opti-MEM (+5%
FBS) in a T-75 flask.
Yuki Ohigashi
