Hello guys!
I need some help about insulin signalling Ab's: we used anti-p85 (PI3K
regulatory subunit), anti-IRbeta and anti-IRS1 for inmunoprecipitation,
but their results are not satisfactory. Someone know GOOD commercial
Ab's for this? It´s very important their immunoprecipitant suitability.
Another question: some tricks for to make better immunoprecipitations on
tyr-phosphorylation experiments? Please, help me, I'm desperate.
Carmen (alvarez at cica.es)