Lipid and T-cell viability

[tosz s. wolf]

Hi,
did you try to cut the lympnode into little peaces before pressing throug a
maize into RPMI? If not, the cells will be squeezed and die. If you store
the T-cells more than 24 hours you shoud use cRPMI (10%fetal cow serum and
1% Penstrep and 1% glucose). Do not use NaOH (cells may burst) use RPMI or
cRPMI for restoring.
regards
Susanne