Sigma sells a "Dissociation Buffer" that serves as a non-enzymatic method
of dislodging cells from plastic surfaces. Give that a try.
Regards,
JPC
Jose Santos Alvarez <alvarez at cica.es> wrote in article
<361DCB1E.95FC1EC0 at cica.es>...
> I need to analyze an adherent cell line for flow cytometry. How to
> detach these cells, without damaging surface proteins? Mecanical
> detachment? EDTA (without trypsin)?
> Thank you for all,
> Jose (alvarez at cica.es)
>> Jose Santos
> Dept. Bioquimica Medica
> Universidad de Sevilla
> Spain
>>>>>>
