IUBio

Panning ab to deplete human hematopoietic cells

Edmund C. Lattime, Ph.D. LattimEC at umdnj.edu
Fri Aug 6 17:25:12 EST 1999


We are trying to purify (enrich) epithelial tumor from lymphoid/myeloid
contaminants.  I would like to do a crude panning step with antibody(s)
that would grab hematopoietic cells.  Rather than add CD3, anti-Ig etc,
has anyone experience with a pan-hematopoietic Mab that works for
panning?
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