kay wrote:
> I need a method of culturing human PBMC's such that they are stimulated
> to proliferate in a non-biased way, i.e. do not prefer either Th1 or
> Th2. In this way, I can determine what my stimulants are doing. To
> respond, remove the x from my name.
> K
I'm not sure what you are asking but i would guess that anti-tcr, anti-cd3,
or a mitigen like con-a would work. Maybe try the PMA, Ca ionophore also
markH
