Moaks wrote:
> Attempting to do a Western on human serum for a novel cytokine.
> Electroblotting to nitrocellulose membrane followed by chemiluminescent
> detection. Problem is that some days (same samples) the candidate band
> is there, others it's not. The problem doesn't seem to be transfer as my
> markers appear to transfer just fine. Also, the detection system is fine
> because I can readily detect recombinant material run on the same gel.
> Any help?
If it's the exact same sample (from the same vial) then it's confusing why
you would see it one day and not the next when all other protocols are
followed exactly and the exact same reagents are used. but deviations from
a protocol could effect results (eg the gel well wasn't as large so the
sample volume is decreased)
markH
