To whom it may concern;
I am a Harvard Medical School Research Fellow with 2+ years of
postdoctoral experience seeking an industrial position in Immunology.
A little about my background....While earning my Ph.D. in Immunology at
Johns Hopkins University School of Medicine in the laboratory of Robert
F. Siciliano, I was directly involved in a Phase I clinical trial
evaluating the immunogenicity and the induction of HIV-1
envelope-specific CTL responses in human volunteers by a canarypox
vector. My continued interest in the development of an HIV vaccine led
me to the laboratory of Norman Letvin where I have worked extensively in
the SIV – rhesus monkey model and with the emerging soluble MHC Class I
tetramer technology. As a Research Fellow at Harvard Medical School I
have worked closely with several large pharmaceutical companies
evaluating candidate HIV vaccines.
My published research has been primarily in the area of cellular immune
responses to experimental HIV vaccines, however, because of my training,
I feel I am capable of contributing significantly to research programs
in any area of basic Immunology, both in humans and in animal models.
I have included a copy of my resume detailing my training and listing my
published work Please feel free to contact me directly with any
questions
Michael A. Egan
==============================================================
Work Address:
Michael A. Egan, Ph.D.
Division of Viral Pathogenesis RE-118
Beth Israel Deaconess Medical Center
Harvard Medical School
42 Avenue Louis Pasteur
Boston, MA 02215
Phone: 617-667-0526
Fax: 617-667-8210
e-mail: michael_egan at caregroup.harvard.edu
Home Address:
154 Pleasant St.
Watertown, MA 02472
617-924-2419
EDUCATION
1987-1992 B.S., Chemistry, Magna Cum Laude, Northern
Arizona University, Flagstaff, AZ
1992-1997 Ph.D, Graduate Program in Biochemistry, Cellular
and Molecular Biology (BCMB), Johns Hopkins University, School of
Medicine, Baltimore, MD
HONORS AND AWARDS
1991 Golden Key National Honor Society
1991 Phi Lambda Upsilon Chemistry Honor Society
1992 Phi Kappa Phi Honor Society
1998-2000 Postdoctoral Training Fellowship in AIDS
Research, Dana-Farber Cancer Institute (NIH)
WORK EXPERIENCE
1997 - present Research Fellow, Division of Viral Pathogenesis, Beth
Israel Deaconess Medical Center, Harvard Medical School
1991 NSF Research Fellowship, University of Georgia
RESEARCH EXPERIENCE
1988-1992 Studied the effect of endurance training on
glucose metabolism.
Laboratory of Dr. Scott Savage and Dr. Hans M. Gunderson,
Exercise Physiology Laboratory, Northern Arizona University
1991 Mutagenesis of murine ferrochelatase in studies of
molecular mimicry in erythropoietic protoporphyria.
Laboratory of Dr. Harry A. Dailey,
Department of Microbiology, University of Georgia
1991-1992 Used semi-empirical computational methods to
calculate the structure and electronic properties of the
mono-boro-analogs of acetylcholine.
Laboratory of Dr. Robert W. Zoellner,
Department of Chemistry, Northern Arizona University
1992-1997 Elucidating the role of the YXXL internalization
motif within the envelope protein (gp41) of HIV-1 in viral assembly,
immune recognition, and CD4 cell loss. Developed a novel method, now
widely used in the AIDS field, for the detection of (HIV-1)-specific CTL
responses in human vaccine recipients. In a Phase I clinical trial
evaluated the ability of an experimental HIV-1 vaccine to elicit
HIV-specific CTL responses in sero-negative volunteers.
Laboratory of Robert F. Siliciano,
Department of Medicine, Johns Hopkins University School of Medicine
1997-present Fine mapped Mamu-A*01-restricted, nondominant,
CTL epitopes in SIV- and SHIV-infected rhesus monkeys. Constructed
soluble MHC Class I tetramers and characterized CD8+ CTL responses to
dominant and non-dominant epitopes in chronically infected rhesus
monkeys. Coordinated a large animal trial assessing SIV gag DNA vaccine
elicited immune responses in rhesus monkeys.
Laboratory of Dr. Norman L. Letvin,
Division of Viral Pathogenesis, Harvard Medical School
PUBLICATIONS
1. Michael A. Egan and Robert W. Zoellner. Structural and Electronic
Characteristics of the Monoboro-Analogs of the Acetylcholine Cation As
Determined by the Semiempirical MNDO Computational Method. J. Org.
Chem., 58:1719-1729 (1993).
2. M.N. Lubaki, Michael. A. Egan, Robert. F. Siliciano, Kent. J.
Wienhold, and Robert. C. Bollinger. A Novel Method for the Detection and
Expansion of HIV Type 1-Specific Cytolytic T Lymphocytes. AIDS Res. Hum.
Retroviruses, 10:1423-1427 (1994).
3. Michael A. Egan, Wendy A. Pavlat, James Tartaglia, Enzo Paoletti, K.
J. Wienhold, Mary Lou Clements, and Robert F. Siliciano. Induction of
Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Cytolytic T
Lymphocyte Responses in Seronegative Adults by a Nonreplicating,
Host-Range-Restricted Canarypox Vector (ALVAC) Carrying the HIV-1MN env
Gene, J. Infect. Dis., 171:1623-1627 (1995).
4. Robert. C. Bollinger, Michael. A. Egan, Tae-Wook Chun, Bonnie
Mathieson, and Robert F. Siliciano. Cellular Immune Responses to HIV-1
in Progressive and Non-Progressive Infections. AIDS, 10:1-12 (1996).
5. Michael A. Egan, Lucy M. Carruth, Jennifer F. Rowell, Xiaofang Yu,
and Robert F. Siliciano. HIV-1 Envelope Protein Endocytosis Mediated by
a Highly Conserved Intrinsic Internalization Signal in the Cytoplasmic
Domain of gp41 Is Suppressed in the Presence of the Pr55gag Precursor
Protein, J. Virol., 70:6547 (1996).
6. Michael A. Egan, Marcelo J. Kuroda, Gerald Voss, Jörn E. Schmitz,
William A. Charini, Carol I. Lord, Meryl A. Forman, Norman L. Letvin.
Use of major histocompatability complex class I/peptide/?2m tetramers to
quantitate CD8+ cytotoxic T lymphocytes specific for dominant and
nondominant viral epitopes in simian-human immunodeficiency
virus-infected rhesus monkeys. J Virol 1999; 73:5466.
7. Christopher B. Buck, Xuefei Shen, Michael A. Egan, Theodore C.
Pierson, Christopher M. Walker, and Robert F. Siliciano. The HIV-1 gag
Gene Encodes an Internal Ribosome Entry Site. Cell 1999; Submitted.
8. Michael A. Egan, Marcelo J. Kuroda, Jörn E. Schmitz, Paul Racz, Klara
Tenner-Racz, Kelledy Manson, Michael Wyand, John W. Shiver, and Norman
L. Letvin. SIV gag DNA Vaccinated Rhesus Monkeys Develop Secondary CTL
Responses and Control Viral Replication Following Pathogenic SIV
Infection. J. Virol., 1999; Submitted.
REFERENCES
Norman L. Letvin, M.D.
Professor of Medicine, Department of Medicine
Division of Viral Pathogenesis Research East Room 103
Beth Israel Deaconess Medical Center
Harvard Medical School
42 Avenue Louis Pasteur
Boston, MA 02215
Office: 617-667-2766 Fax: 617-667-8210
E:mail: nletvin at caregroup.harvard.edu
Robert F. Siliciano, M.D., Ph.D.
Assistant Professor, Department of Immunology
Johns Hopkins University School of Medicine
Rm. 1049, Ross Research Bldg.
720 N. Rutland Ave.
Baltimore, MD 21205
Office: 410-955-2958 Fax: 410-955-0964
e-mail: rsilcia at welchlink.welch.jhu.edu
Robert C. Bollinger, M.D., M.P.H.
Assistant Professor, Division of Infectious Disease
Johns Hopkins University School of Medicine
Rm. 1159, Ross Research Bldg.
720 N. Rutland Ave.
Baltimore, MD 21205
Office: 410-614-0936 Fax: 410-955-7889
e-mail: rcb at welchlink.welch.jhu.edu
