IUBio

question about HIV floecytometry

mark mark.haynes at mail.tju.edu
Wed Feb 3 16:54:28 EST 1999


Lixin Wang wrote:
> 
> Need help
> Here is the experiment design: primary culture of human
> cells(adherent,mixed types of cells), will use dispase to dissociate the
> cells, and then stain the cells for two or three color flow, one is for
> HIV antigen expression,now I hope some one can help me to find which
> antibody I can use against HIV,from where.
> Highly appreciated!
> e-mail:  lwang1 at mailhost.tcs.tulane.edu

Hi
I would suggest that you talk with individuals in the pathology lab in 
the hospital associated iwth tulane.  ALso you might want to talk to the 
flow people regarding abs and also the clean-up procedures for the 
machine.
markH



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