Isolation Monocytes

[Conny Stuelten]

I am trying to isolate monocytes from human PBMC with density gradients. In
the beginning I have used OptiPrep-gradient and obtained a
purification from 60%. Now I utilize an Iopromid-gradient (Biochrom) with
the density 1,068 g/l, but I got no results (no seperation). Has anyone
worked with Iopromid-gradient or with another Monocyte-gradient?

Thanks Conny