Eric Park wrote:
>> I'm encountering some problems when I try to isolate neutrophils from my
> blood. I'm using a Ficoll-Hypaque solution when I centrifuge the blood.
> This technique works great for several other people but not for me. A lot
> of RBCs are remaining in the neutrophil layer and not settling down to the
> bottom of the tube. Does anyone know why this is happening to my blood?
>> Personal info:
> Blood type is B+ Rh+
> Asian male 22 years old
>> Insights would be helpful since this is a mildly annoying problem in my
> research by begging for volunteers instead of tapping my own blood.
In the world of flow cytometry, many technologists utilize ficol
histopaque medium to recover lymphocytes from anticoagulated whole blood
or lymphocytes from lymph nodes. Spun at 1500 rpms for 30 minutes
yields an excellent recovery of these T and B cells with very little
monocyte or other leukocyte contamination.
