In article <79v8df$8nv$1 at nclient3-gui.server.virgin.net>, "Andrew Hall"
<andrew.hall at virgin.net> wrote:
> I came across an unusual phenomenon today whilst separating human PBMC from
> whole blood over Ficoll Hypaque. After removing the buffy layer and spinning
> it down the supernatant appeared to solidify. I was unable to recover any
> cells from this prep. The supernatant contained serum and MEM alpha medium.
> The serum from the sample (taken separately) was very cloudy. Does anyone
> have any suggestions as to why this should happen.
When was the sample drawn? Lipids accumulate in the serum shortly after
ingestion of fatty food, and this is a common problem in any blood test /
cell purification procedure.
Guy
--------Guy Hermans, PhD student------------
MS research Unit Tel 0032 (0) 11/26.92.07
Dr. L. Willems-Institute Fax 0032 (0) 11/26.92.09
Belgium
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