More than likely what you had was plasma as opposed to serum since you were
using anticoagulated whole blood. That being the case and depending on the type
of anticoagulant you used the plasma could be made to clot by adding excess
calcium which may have come from your MEM media
Andrew Hall wrote:
> I came across an unusual phenomenon today whilst separating human PBMC from
> whole blood over Ficoll Hypaque. After removing the buffy layer and spinning
> it down the supernatant appeared to solidify. I was unable to recover any
> cells from this prep. The supernatant contained serum and MEM alpha medium.
> The serum from the sample (taken separately) was very cloudy. Does anyone
> have any suggestions as to why this should happen.
--
Michael J. Richards
Research Biochemist
