Our lab is trying to find a way to purify B and T cell subsets using
magnetic separation. We are currently using Biomag particles conjugated to
goat-anti rat Ab to make B, CD4+, and CD8+ from mouse spleen and lymph node.
The procedure is faster and cheaper (the magnet is much smaller and it
accomodates 6 microcentrifuge tubes, 1 15cc tube or 1 50cc tube) than the
MACS system but the purity and yield is not optimal. Does anyone have any
tips to increase the purity or the yield of this method?
