Chen, Peter wrote:
>> Our lab is trying to find an efficient method to purify B cell populations
> from the spleen using magnetic beads or panning techniques. Is there an easy
> way to get rid of the myeloid cell populations? (we have found that
> macrophages make up only a small percentage of this cell population and
> using Ab to select them out would not increase the B cell purity
> significantly). Thanks.
Magnetics would be a good choice. I'm sure papers by kelsloe or kelso or
something, or time mansers group would have some techinical avenues for
you to read about