Try treating the BMDC with TNF-alpha, LPS or cross-linking CD40. This should
activate and mature your BMDC. B7.1 expression is often not very high
(depending upon your antibody of course!) while B7.2 can be much higher.
Tim Bullock; tb5v at virginia.edu
Geoffrey M Thiele wrote:
> I have made dendritic cells by methods found in current protocols (mouse BM
> cells and GM-CSF). I want to use the cells to find the upregulation of B7
> expression. The cells appear to be immature (low class II expression) and
> further subculturing does not increase this. The cells do express CD11c a
> great deal. Any suggestions as to solving the problem of immature cells?
