All-
We are in the process of setting up production of CY-3 labled IgG2
antibodies. We are rather unexperienced in this technique and need any
help we can get. Our specific questions revolve around: 1) how purified
the monoclonals must be prior to lableling, 2) what methods of
purification are best, 3) what are the best kits or generic methods for
labling (at this point, we intend on trying research organics kit), 4)
what advice do you have for setting up the cell lines, harvesting
monoclonals, etc. We hope to find a safe, rather idiot-proof set of
protocols. Thank you in advance for any help or references you may
give. Thanks again.
Scott Meschke
