I have a problem in doing immunohistofluoresence. I have non-fixed
cryosections of inflamed mouse knee joints (the bone tissue is not
decalcified). They are air dried and frozen at -70°C. The problem is that
the antibodies, which work on acteone fixed and non-fixed spleen
cryosections, obviously do not bind to the epitopes. I don't get any
staining neither in the joint region nor in the bone marrow. I also tried
several amplification methods with secondary and tertiary
reagnets/antibodies. Does someone has any experience with that type of
tissue? Is it possible that components of the synovial fluid block antibody
binding?
I would appreciate any help.
Thanks
FRANK HARDUNG
