Paula Lavery wrote:
>> We are trying to isolate RNA from patients' blood samples. So far, I have
> been lysing RBC from buffy coats with ammonium chloride solution and storing
> the WBC in DEPC-PBS at -70C. (RNA isolation is done later in another lab.)
> The RNA yield is very low from these samples. Is anyone else isolating RNA
> from blood? I'd appreciate any help or hints with this. Thanks!
Why not try doing it sequencially to see if the storage is the problem
Also what is the actual yield
is it polyAAAAAAAAA or total?
If total how distnct are the ribosomal bands after gel electorphoresis?
have you tried rt-pcr with a ubiquitous gene like gapdh?
markH
