Why don't you use a commercially available product such as TRI REAGENT by
Molecular Research Center Inc. which is designed to isolate RNA, DNA and protein
from the same sample. I have been told by local people that it is easy to use
on blood samples. We have used similar products with success on liver samples.
Simon Worrall
Lecturer in Biochemistry
University of Queensland
Australia
Paula Lavery wrote:
> We are trying to isolate RNA from patients' blood samples. So far, I have
> been lysing RBC from buffy coats with ammonium chloride solution and storing
> the WBC in DEPC-PBS at -70C. (RNA isolation is done later in another lab.)
> The RNA yield is very low from these samples. Is anyone else isolating RNA
> from blood? I'd appreciate any help or hints with this. Thanks!
