IUBio

RNA from blood

oliveira at netra.cpqrr.fiocruz.br oliveira at netra.cpqrr.fiocruz.br
Wed Jul 28 08:40:56 EST 1999


Dear Paula,
we have used Qiagen's line of kits for extracting both DNA and RNA from
a variety of samples very successfully.  I'd give it a shot,
Good luck
Guilhere

In article <lK%m3.13966$8v6.678204 at carnaval.risq.qc.ca>,
  "Paula Lavery" <plaver at po-box.mcgill.ca> wrote:
> We are trying to isolate RNA from patients' blood samples. So far, I
have
> been lysing RBC from buffy coats with ammonium chloride solution and
storing
> the WBC in DEPC-PBS at -70C. (RNA isolation is done later in another
lab.)
> The RNA yield is very low from these samples. Is anyone else isolating
RNA
> from blood? I'd appreciate any help or hints with this. Thanks!
>
>


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