We have found that cells stored in this manner tend to degrade the RNA after
a few weeks to months. We have found it to be more stable if we lyse them
in Tri-reagent (a commercial variant of the acid-phenol extraction lysis
buffer) and then store them at -70C.
-james-
--
James F. George, Ph.D.
Rm 739 ZRB
Department of Surgery
University of Alabama at Birmingham
Birmingham, AL 35294-0007
Homepage: Http://www.uab.edu/transplantimmuno
205-934-4261 Voice
205-934-5261 FAX
Paula Lavery <plaver at po-box.mcgill.ca> wrote in message
news:lK%m3.13966$8v6.678204 at carnaval.risq.qc.ca...
> We are trying to isolate RNA from patients' blood samples. So far, I have
> been lysing RBC from buffy coats with ammonium chloride solution and
storing
> the WBC in DEPC-PBS at -70C. (RNA isolation is done later in another lab.)
> The RNA yield is very low from these samples. Is anyone else isolating RNA
> from blood? I'd appreciate any help or hints with this. Thanks!
>>
