Katherine Liza Kravik wrote:
>> I am staining paraffin-embeded tissue. Instead of microwaving which loosens
> the tissue from the slides I've thought of using a permeaabilisation
> solution (0.1% Triton X-100 in 0.1% sodium citrate for 8 min). Does any one
> have a receipe for the above mentioned solution? Does anyone have any
> experience with this permeabilisation solution?
Katherine, what are you trying to accomplish? I know microwave treatment is
one way of uncovering ags for staining and there are other ways. both zymed
and vector sell a citrate buffer that (i think) is used as a hot water bath
treatment for the same purpose.
If your sections are falling off other approaches are treated slides with a
number of things I've used poly-l-lysine (ok) and cell-tak (better) now i use
super frost plus slides (good).
Permeabilizations that i know of have used saponin, coulter and BD sell
solutions, you can also make your own the reference is B. Sander et
al,mImmunol. Rev. 1991, #119 p65
