I am trying to sort out naive T cells (CD45RA+). This is a subset of
PBMC,of CD3+T cells of CD3+CD4+ T cells. Last time, I did not get
enough cells from 17ml of blood for my stimulation experiment ( I need
at least 2,500,000. I need to know the number of lymphocytes in say a
ml of blood and the percentage of each subset I should expect out of
that number. That way I can save myself some BLOOD, sweat and tears.
