On Tue, 02 Mar 1999 11:15:38 -0400, "Chidi"
<ngwu at helix.mgh.harvard.edu> wrote:
>Hi,
>>Has anyone tried subcloning hybridomas with a FACS sorter? I want to get
>away from manual single cell picks and serial dilutions. It will be nice to
>throw in recently fused cells (B cells to Myeloma fusions) and sort them
>into singles.
>--
>Chidi
>>-- Do unto others as you will have them do unto you --
Dear Chidi
People have been doing this for about twenty years so there's lots of
experience. I've done it with a BD Facstar+ and the BD single-cell
deposition attachment. Some hybridomas, e.g. NS-1 based, are more
robust than others. Newly-fused cells are very fragile and anyway you
want to do the usual growth on selective media BEFORE sorting to get
rid of the large number of cells that will die.
There are lots of relevant resources on the web. Start with
www.flowcyt.cyto.purdue.edu
about the best single subject-specific resource I know of in any of
the scientific areas I've scanned for on the Internet.
Best Wishes
Terry Prospero
terryprospero at hotmail.com
