Boehringer Mannheim has a product called Fugene 6 which works very well
with primary cell cultures. For a protocol try current protocols in cell
or molecular biology. Good luck!
On Mon, 1 Mar 1999, Ian Mc wrote:
> Hi,
>> Does anyone have a protocol for transiently transfecting B-lymphocytes
> from human blood ? Or could you suggest a reference or a product which I
> could use.
> I tried searching medline but only got a thousand and one articles on
> b-cell lines.
>> Thanks
>> Ian Mc
>>