Derek Gray wrote:
However, I am interested in
> using higher concentrations of the serum and at concentrations of 20
> percent or higher the calcium concentration will be way outside the
> normal range. So far it looks as though this promotes even higher
> activation of T-cells. Interestingly I cannot find any publication
> listing the effect of high extra-cellular calcium concentration on T
> cell activation on Medline (I guess relevant publications may pre-date
> Medline), but from the knowledge that calcium influx is crucial to
> T-cell activation I presume the effect would be enhancing.
John Kay at the University of Sussex, did a lot of work on this. See
for example "Interaction of Lymphocytes and PHA: inhibition by chelating
agents" (1971) Experimental Cell Research 68, 11-16.
Sincerely, Donald Forsdyke,
Discussion Leader. Bionet.immunology
