anlong xu wrote:
> The nucleotide sequence were performed to verify one point mutation
> between two PCR products, but our PCR-SSCP can not detect the
> differentiation. I will be grateful if anyone can tell me why.
SSCP detects differences in conformation, so if your point mutation does
not cause a significant change in conformation then it will be
undetectable by SSCP.
Dr Andrew Walley
Asthma Genetics Group,
Wellcome TRust Centre For Human Genetics,
Oxford, UK
