Paula Lavery wrote:
> We are having problems getting FK506 into solution to use in some in vitro
> assays. It readily dissolves in EtOH (0.2M) but it precipitates out whenever
> we try to dilute it further into culture medium. We tried adding some Tween
> 80 to the solution but it didn't help. Anyone have any bright ideas?
>> Paula Lavery
> Transplant Immunology Lab.
> Royal Victoria Hospital
> McGill University
> Montreal (QC) Canada
>> "If we knew what we were doing it wouldn't be called research, would it?"
> -- Albert Einstein
My first thought was "how is it normally done?" and "how are you actually doing
it?"
I used cyclosporine a long time ago and what we used to do is slowly add diluent
(culture fluid etc) to the etoh-csa. Are there other solvent you can use
(DMSO). WHat temp is the culture fluid? does serum play a role either pos or
neg in the precipitating out?
markH
hey i'm alumni at mcgill--great school, great town, (is bob murgita still
there?)
Peace
markH
