I'm doing the Western blot with Amersham ECL
Kit(RPN2106) ,however, the result is very ugly, So I could
only use AP(NBT/BCIP)system, and this system
sensitivity is low. Can someone good at this give me
some know-how? Thanks a lot. By the way, I'm doing immunoprecipitation with
HA- and C-myc tag,and constructed the expression plasmid myself. But
I can not do it well. In the Western blot, I can not
watch the band. Can some specialist help me with this
problem? Thank you very much!!!
Jason
E mail address jxgu at shmu.edu.cn
