Hello all,
When you do flow cytometry or cell sorting using anti-CD19 antibodies
as a pan B cell marker ... does this antibody activate and crosslink the
CD19 molecule on the B cells??
and would this crosslinking have an effect upon mature recirculating B
cells in the absence of anti-Ig stimulation.
I'm thinking about raising an antibody against rabbit CD19 and wanted to
know if polyclonal anti-CD19 would have any effects on the B-cells as
they were sorted using this antibody... if they are all going to undergo
apoptosis using polyclonal serum, I would need to make monoclonal
antibodies and screen them for clones which do not activate CD19 ...
anyone using anti-CD19 (mouse or human) or have any thoughts?
thanks..
Paul
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___ ____ ____ ____ ____________________ ____ ____ ____ _
\ \ / \ \ / \ \ / \ \ / Paul Jasper \ / \ \ / \ \ / \ \ /
/\ \ /\ \ /\ \ /\ Micro/Immuno - Loyola U. /\ \ /\ \ /\ \
\__\/ \__\/ \__\/ \__pjasper at orion.luc.edu__\/ \__\/ \__\/ \__\/
