Dear Student
We use HiTrap Protein G columns (and HiTrapNHS-mAb columns also) and they seem
to last and last. We have had only one column fail in ~6 years, which was a
Protein G column used to purify mouse asites antibody. As long as the serum
is clean (lipids can kill any column quickly) a HiTrap column use by date can
be regarded as a long time! We have had a HiTrapNHS-anti ovine IgE mAb column
still working well after in excess of 500x pH 3.0 Glycine elutions. Actually
you are more likely to lose a column by dropping it and breaking the end off.
Richard
"Student" <mucineer at iname.com> wrote:
>Does anyone use the HiTrap Protein A columns from Pharmacia to purify human
>IgG? Can they be regenerated for more purifications and how do they perform?
>Approximately how many times can this be done?
>>Many thanks.
>>>>>
Richard Shaw Internet: ShawR at agresearch.cri.nz
Researcher (Science technician)
P.O.Box 40063, Upper Hutt
NEW ZEALAND
Technicians Do Most Things Better!!
Always be Happy and Just Do It!! (to coin a well Known slogan)
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Disclaimer: The above is a personal opinion and does not reflect the
official view of AgResearch Ltd.
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