An alternative method may be to get the macrrophage cell line J774. They
still have there Fc receptors and can be used for opsonization studies.
We are currently setting up this system in our lab.
Paul Cullen
Bacterial Pathogenesis Research Group
Monash University
Tony Schountz wrote:
>> I'm having a bit of a problem that I hope someone might be able to help
> me with. I'm trying to harvest peritoneal macrophages from deer mice,
> but the cells that I get do not look like macrophages because they do
> not adhere to tissue culture plates. My protocol is to inject deer mice
> and BALB/c with 1.5 ml of 3% thioglycolate then harvest 3-5 days later.
> The BALB/c clearly give us macrophages, but the deer mice do not. Any
> suggestions for alternative macrophage harvesting?
>> Thanks,
>> Tony
>> --
> Tony Schountz, Ph.D.
> Department of Biological Sciences
> Mesa State College
> mailto:tschount at mesastate.edu
