Hi,
I am trying to attempting to stain a population of periferal blood with
a few different lineage panels including Erythroid for flow cytometric
analysis. I am finding , however that my Glycophorin A stain is too
bright even when titrated down to 1µl/lamda. I suspect nonspecific
binding. Does anyone know of a method to block binding using ADP?
Thanks.
Paul Fallon