Paul
If your Glycophorin A is a direct conjugate, you should dilute it with
unlabeled Glycophorin A, thus reducing the signal but still saturating
the sialoglycoprotein with antibodies.
What is 1 microliter per lambda? Erythrocytes are bright for Glycophrin
A, so 0.01 micrograms of antibody per microliter of blood is not
outrageous.
"Fallon, Paul G." wrote:
>> Hi,
> I am trying to attempting to stain a population of periferal blood with
> a few different lineage panels including Erythroid for flow cytometric
> analysis. I am finding , however that my Glycophorin A stain is too
> bright even when titrated down to 1µl/lamda. I suspect nonspecific
> binding. Does anyone know of a method to block binding using ADP?
> Thanks.
>> Paul Fallon
--
---Dennis
Dennis J. Young
Flow Cytometry Core Facility
University of California, San Diego
