I am trying to identify integral membrane proteins
from human cancer cells recognized by monoclonal
antibodies that we have developed. We are
performing immunoprecipitation, separating the
proteins by SDS-PAGE, performing in-gel digestion
with trypsin and sending the samples for MALDI-TOF
analysis. Many of the proteins are glycoproteins.
Do we need to remove the carbohydrate chains
sometime before MALDI-TOF analysis?
Our main purpose is identification of the proteins
(antigens) rather than studying post-translational
modifications.
Thanks,
Steve Roffler
Institute of Biomedical Sciences
Academia Sinica
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