IUBio

FACS: excluding dead cells?

maciej simm simm at cd4cd8.com
Sat Feb 5 21:15:42 EST 2000


someone once suggested using PI but I think that leaks into PE channel as
well as
far red.. Not sure if it's worth it. But if you have an extra tube one day
:)

Maciej

Lionel Berthoux wrote:

> Hi,
>
> I am FACscanning cells using GFP + a PE-labeled Ab. I would like to
> exclude dead cells. Do you know of any reagent that would be useful in
> that case? (I think 7AAD is not compatible with the use of PE)
>
> thank you
>
> Lionel lb8x at virginia.edu





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